|
1. Expression of target proteins
|
| ● |
Escherichia coli expression system |
| ● |
Yeast expression system |
| ● |
Mammalian cell expression system |
| ● |
Escherichia coli expression system specialized for membrane protein |
|
 |
| |
|
2. Fine analysis on physical and chemical properties of target protein
|
| ● |
Circular dichroism |
| ● |
Analytical ultracentrifuge |
| ● |
Biacore |
| ● |
Isothermal titration calorimetry |
| ● |
uclear magnetic resonance |
| ● |
Dynamic light scattering |
|
|
| |
|
3. High-resolution NMR analysis on Physical and Chemical Properties of the target protein
|
| ● |
Rapid evaluation on protein structures via 1D or 2D 15N-HSQC NMR |
| ● |
Detection of weak binding between protein-protein or protein-small molecule interactions |
| ● |
Structural determination of protein-bound ligands via STD NMR or transferred NOE |
| ● |
Protein dynamics by NMR |
| ● |
Measurement of side chain pKa values in proteins |
| ● |
Lead compound/drug screening and optimization |
|
|
| |
|
4. Identification of target protein
|
| ● |
Identification of individual protein within protein complexes |
| ● |
Identification of protein post-translational modifications |
| ● |
Whole-proteomic sequencing of antibody or drug target(s) |
| ● |
Glycome analysis of protein glycosylation |
| ● |
Quantitative analysis of protein post-translational modification |
|
|
| |
|
5. 3D structural analysis of target protein
|
| ● |
Protein X-ray crystallography |
| ● |
Nuclear Magnetic Resonance Spectroscopy (NMR) |
| ● |
Cryo-electron microscopy (Cryo-EM) |
| ● |
Small-angle X-ray scattering (SAXS) |
|
|
| |
|
6. Experimental structure-based protein engineering and drug design
|
| ● |
Structure-based design and optimization of small-molecule drug |
| ● |
Structure-based protein engineering |
| ● |
Structure-based antibody engineering |
|
|
| |
|
7. Computer-aided drug design and systems biology
|
| ● |
Virtual screening |
| ● |
Molecular docking |
| ● |
Bioinformatics analysis |
| ● |
Structure-based systems biology analysis |
| ● |
High-throughput bioanalysis |
| ● |
Biological network analysis |
|
|
| |
|
8. Advance proteomics analysis
|
| ● |
High-performance proteomic/phosphoproteomic/glycoproteomic profiling platform |
| ● |
Personalized proteomics analysis of clinical specimens |
| ● |
Multiplexed protein quantification by multiple reaction monitoring |
|
